Document Details
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Document Type |
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Thesis |
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Document Title |
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Isolation and identification of some antimicrobial producing bacteria from caves in Summan plateau عزل وتعريف بعض البكتيريا المنتجة لمضادات الميكروبات من كهوف هضبة الصمان |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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During the past decades, some natural environments in the Kingdom of Saudi Arabia have been studied in terms of microbial content, but there are still some environments that have not been studied thoroughly. This study aimed to select the caves as one of these environments, specifically the caves of the Summan plateau located northeast of Riyadh to detect the possibility of the presence of some bacteria with the ability to produce antimicrobials. From eight soil samples were taken from different areas of the cave, the bacterial isolates were obtained on nutrient agar medium and soil extract medium and after the development of microbial cultures and the purification of microbial isolates, 56 isolates were obtained. The ability of all isolates to antagonize with some pathogenic bacteria, yeasts and fungi was studied. Two of the most antagonistic effect isolates AC30 and AC32 were selected to apply further experiments, as the greatest effect was against Staphylococcus aureus bacteria, it was chosen to estimate the antagonism of the two isolates while studying the effect of the variables on them. The two isolates were cultivated on different media and the best medium for AC30 isolate growth and best antagonist result was the nutrient broth medium while the sucrose nitrate medium was the best for the antagonist result of isolate AC32 and the best media for the growth was nutrient broth medium (NB). The effect of different factors such as the best carbon source, the best nitrogen source, the best pH value, the best temperature and the best incubation period on the isolation growth and the efficiency of its activity against microbes were studied. The result of the effect of different factors respectively on the growth of isolate AC30 were glucose, yeast extract in (NB)., pH5, 37 °C and 24 hrs., and on the growth of isolate AC32 are sucrose, sodium nitrate NaNO3, pH 9, 35 ° C, 72 hrs. Their effect on antagonism was found to isolate AC30 as glucose, yeast extract in (NB), pH 7, 37 ° C, 48 hrs. For isolation AC32 are sucrose, NaNO3, pH 6, 35 ° C, 48 hrs. Next, the microbial identification of the two selected bacterial isolates was carried out. Morphology and physiological and biochemical characteristics were determined. The two isolates were genetically identified by 16S rRNA, where the first isolate belong to the species Paenibacillus polymyxa and the other was defined as Paenibacillus peoriae, it is one of the new species. |
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Supervisor |
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Dr. Reda HassanHussein Amasha |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1441 AH
2020 AD |
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Co-Supervisor |
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Dr. Samyiah Darwish Saddig Jastaniah |
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Added Date |
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Saturday, June 6, 2020 |
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Researchers
| أمل حسان العنبري | Alanbari, Amal Hassan | Researcher | Master | |
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